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KMID : 0545120010110020333
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.333 ~ p.336
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Production of P(3-hydroxybutyrate-3-hydroxyvalerate) and P(3-hydroxy-butyrate-4-hydroxybutyrate) Using Transformant Alcaligenes latus Enforcing Its Own phbC Gene
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Seo, Il Seon
Jung, Young Mi/Lee, Yong Hyun
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Abstract
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An isolated phbC gene from Alcaligenes latus was reintroduced into the parent A. latus through the transformation process, and the effect of the amplified phbC gene on the biosynthesis of P(3-hydroxybutyrate-3hydroxyvalerate) [P(3HB-3HV)] and P(3-hydroxybutyrate-4-hydroxybutyrate) [P(3HB-4HB)] in the transformant A. latus was investigated. The biosynthesis rate and content of the above copolymers increased up to 1.3-fold after enforcing its own phbC gene, and the molar fractions of 3HV and 4HB in P(3HB-3HV) and P(3HB-4HB) also changed remarkably from 35.0 to 48.0% and from 34.0 to 56.0%, respectively, showing a critical role of PHB synthase which catalyzes the polymerizing reactions between either 3HV or 4HB from precursor compounds and 3HB.
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